Dr. Ann Miller Associate Professor Department of Molecular, Cellular, and Developmental Biology (MCDB) Member, Cellular and Molecular Biology Program (CMB) Address University of Michigan 5264 Biological Sciences Building
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Weimin Sun Ph.D., ABMG, CGMB, CLSP(MB), in Molecular Diagnostics, 2010 Salting-Out Method As an alternative to the phenol-chloroform extraction procedure, the salting-out method has the advantage of not using toxic chemicals.
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Zirconium Molecular Biology Grade beads diameters of 100 µm, 200 µm, 400 µm, 800 µm, 1.0 mm or 1.4 mm are offered as pre-filled tubes; silica Molecular Biology Grade beads in pre-filled tubes have diameters of 100 µm, 400 µm or 800 µm are available in pre-filled tubes.
Read 4 answers by scientists with 2 recommendations from their colleagues to the question asked by Ian Lidbury on Jun 13, 2013
Leah M. Miller, ... Pascal Verdier-Pinard, in Methods in Cell Biology, 2010. c Discussion. Tubulin isolated from chicken erythrocytes is composed of only one α- and one β-tubulin isotype, ... Koji Ikegami, in International Review of Cell and Molecular Biology, 2012. 4.3.2 Acetylation.
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Optional Kryo-Tech Accessories enable cryogenic grinding and preserve temperature sensitive samples for RNA and protein extractions. Optional Large Clamp Assembly holds four deep-well titer plates, multiple centrifuge tubes, and other large grinding vials.
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Molecular Biology; Open Reading Frame (ORF) PCR & Amplification; ... Reagents for Grinding and Tabletting. Spectromelt® Reagents for Grinding and Tabletting Optimized for Automated Sample Preparation Tabletting the sample is an alternative sample preparation for XRF to fusion. The Spectromelt® C-series is...
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Three grades of grinding beads are produced by OPS Diagnostics, and include Acid Washed, Low Binding, and Molecular Biology Grade. Acid Washed grinding beads are washed with acid to remove small particles and contaminants from the media then oven …
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Cell grinding using the cryomilling machine takes ∼3 hr. Protein purification requires ∼8 to 9 hr for affinity chromatography, another ∼8 to 9 hr for gel‐filtration chromatography, and ∼1 to 3 hr for concentrating the recombinant protein.
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Centre for Cellular and Molecular Biology; What is the role of 300 mM NaCl used to prepare buffers used for protein samples? ... In general 300 mM is about double ionic strength and osmolarity ...
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E. coli Plasmid DNA MiniPrep Protocol. Introduction. The isolation of plasmid DNA from E. coli using an alkaline lysis is a well-established method.E. coli with plasmid is cultured in media with antibiotics to a high cell density, harvested, and then lysed with a SDS/NaOH solution. Rapid acidification using concentrated potassium acetate causes the precipitation of protein and chromosomal DNA.
Professional Interests and Activities: Teaching and research in structural molecular biology, biochemistry, and biophysics. Experimental, computational, and theoretical methods of biocrystallography. Experimental, computational, and theoretical methods of biocrystallography.
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Plant Molecular Biology includes products for Plant Nucleic Acid Purification, Nucleic Acid Amplification, Genome Mapping and Genotyping, Sequencing, Cloning, Gene Expression Analysis, Nucleic Acid Electrophoresis and Reagents for Nucleic Acid Purification.
The choice of grinding tools and accessories ensures that our instruments provide for contamination-free and reliable sample preparation prior to laboratory analysis.